Development of species-specific real-time PCR assays for the identification of five European Rhinolophus bats
Identification
- Clé publique
- radar:r-90b58d0da2d8309bbd20b573423c5764
- Auteurs
- Patrick G. R. Wright · Marina Bollo Palacios · Thomas Kitching · Daniel Hargreaves · Szilárd‐Lehel Bücs · Ivana Budinski · Branka Bajić · Csaba Jére · István Csősz · Isabella Campbell Harry · Thomas J. Etheridge · Fiona Mathews
- Année déclarée
- 2026
- Langue déclarée
- en
- Type source
- preprint
- Première détection
- 2026-11-14T02:30:08.088326+00:00
- Dernière observation des métadonnées
- 2026-11-14T02:30:08.088326+00:00
- Dernière vérification HTTP
- Non effectuée
Institutions déclarées par la source
Vincent Wildlife Trust · University of Belgrade · Institute for Biological Research “Siniša Stanković” · University of Sussex
Pays d’affiliation déclarés
GB · RS
Résumé documentaire — langue originale
Abstract The detection and monitoring of bat species using non-invasive sampling and molecular techniques has become increasingly popular in recent years. In Europe, these approaches have been applied to identify horseshoe bats of the genus Rhinolophus , which includes five species: R. hipposideros, R. ferrumequinum, R. euryale, R. mehelyi and R. blasii . While species-specific real-time PCR assays exist for R. ferrumequinum and R. hipposideros , no unified panel of real-time PCR assays currently enables the identification of all five European Rhinolophus species from non-invasively collected samples. Here, we developed five species-specific real-time PCR assays, each targeting interspecies nucleotide variation within the mitochondrial cytochrome b gene. To enhance single-base discrimination, RNase H-dependent PCR (rhPCR) primers were employed, incorporating cleavable blocked primers that require perfect complementarity for extension. The assays were applied to droppings non-invasively collected from 18 caves and one church in Serbia and Romania. Of the 149 samples analysed, 131 (88%) yielded successful amplification of Rhinolophus DNA. Detection probabilities for the three species identified in the field ranged from 0.49 to 0.82. Occupancy estimates varied, with R. euryale showing the highest (0.86; UI: 0.69–0.97) and R. mehelyi the lowest (0.23; UI: 0.08–0.43). The assays were capable of detecting up to three species concurrently within a single pooled sample (approximately 15 droppings). These assays are especially valuable for detecting R. mehelyi , given its rarity and uncertain distribution, and offer a robust tool for monitoring Rhinolophus populations across Europe.
Identifiants
- OPENALEX
- https://openalex.org/W7155371310
- DOI
- 10.64898/2026.04.21.719147
Repères documentaires
- Famille documentaire
- preprint
- Types déclarés par les sources
- preprint
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Sources
OpenAlex · Document 1
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Patrick G. R. Wright; Marina Bollo Palacios; Thomas Kitching; Daniel Hargreaves; Szilárd‐Lehel Bücs; Ivana Budinski; Branka Bajić; Csaba Jére; István Csősz; Isabella Campbell Harry; Thomas J. Etheridge; Fiona Mathews. Development of species-specific real-time PCR assays for the identification of five European Rhinolophus bats. 2026. https://doi.org/10.64898/2026.04.21.719147
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- APA
- Patrick G. R. Wright; Marina Bollo Palacios; Thomas Kitching; Daniel Hargreaves; Szilárd‐Lehel Bücs; Ivana Budinski; Branka Bajić; Csaba Jére; István Csősz; Isabella Campbell Harry; Thomas J. Etheridge; Fiona Mathews. (2026). Development of species-specific real-time PCR assays for the identification of five European Rhinolophus bats. https://doi.org/10.64898/2026.04.21.719147
- Chicago
- Patrick G. R. Wright; Marina Bollo Palacios; Thomas Kitching; Daniel Hargreaves; Szilárd‐Lehel Bücs; Ivana Budinski; Branka Bajić; Csaba Jére; István Csősz; Isabella Campbell Harry; Thomas J. Etheridge; Fiona Mathews. Development of species-specific real-time PCR assays for the identification of five European Rhinolophus bats. 2026. https://doi.org/10.64898/2026.04.21.719147
- ISO 690
- Patrick G. R. Wright; Marina Bollo Palacios; Thomas Kitching; Daniel Hargreaves; Szilárd‐Lehel Bücs; Ivana Budinski; Branka Bajić; Csaba Jére; István Csősz; Isabella Campbell Harry; Thomas J. Etheridge; Fiona Mathews. Development of species-specific real-time PCR assays for the identification of five European Rhinolophus bats. 2026. https://doi.org/10.64898/2026.04.21.719147
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UDTR — Bibliothèque juridique mondiale des droits de la Nature. Development of species-specific real-time PCR assays for the identification of five European Rhinolophus bats. Notice documentaire: https://www.declarationuniverselledesdroitsdelarbre.org/global-rights-of-nature-radar/reference/r-90b58d0da2d8309bbd20b573423c5764/ · Dernière vérification HTTP : non effectuée
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Source bibliographique / publication d’origine · https://doi.org/10.64898/2026.04.21.719147
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