UDTR — Radar mondial des droits de la Nature

Deciphering global transcriptional dynamics coordinated by gene-gene regulatory interactions using single-cell data

Identification

Clé publique

radar:r-8078af21330954556bbea45a66bc407c

Auteurs

Liying Zhou · Songhao Luo · Zhiwei Huang · Zhenquan Zhang · Zihao Wang · Jiajun Zhang

Année déclarée

2026

Langue déclarée

en

Type source

article

Première détection

2026-11-14T02:03:31.399789+00:00

Dernière observation des métadonnées

2026-11-14T02:03:31.399789+00:00

Dernière vérification HTTP

Non effectuée

Institutions déclarées par la source

Sun Yat-sen University · Guangdong Province Key Laboratory of Computational Science · University of California, Irvine · Guangdong University of Technology

Pays d’affiliation déclarés

CN · US

Résumé documentaire — langue originale

Transcription is an inherently dynamic and stochastic process that often occurs in bursts, governed by gene-gene regulatory interactions and thereby driving cell-to-cell heterogeneity. However, a genome-wide, mechanistic understanding of how regulatory networks globally shape transcriptional bursting dynamics remains lacking. Here, we present BurstLink, an interpretable and tractable statistical-mechanistic framework that simultaneously infers coupled regulatory interactions and transcriptional bursting kinetics at the genome-wide scale from single-cell data. BurstLink introduces reweighted mutual information to quantify regulatory strength as network edge weights, while jointly inferring regulatory directionality and interaction type for each gene pair within a unified mechanistic model of transcriptional bursting. Applied to mouse embryonic fibroblasts data, BurstLink reveals several genome-wide regulatory mechanisms on transcriptional bursting: downstream target genes exhibit higher burst frequency and gene-expression variability than upstream transcription factor genes; stronger transcription factor binding affinity is associated with lower burst frequency and higher burst size of target genes. Notably, positive regulation primarily enhances the burst frequency and gene-expression variability in target genes, in contrast to negative regulation. In summary, BurstLink deciphers multiple general principles of global transcriptional dynamics, providing novel biological insights into cell fate decisions.

Identifiants

OPENALEX

https://openalex.org/W7172303703

DOI

10.1038/s44320-026-00235-4

PMID

https://pubmed.ncbi.nlm.nih.gov/42547851

PMCID

https://www.ncbi.nlm.nih.gov/pmc/articles/13728105

Repères documentaires

Famille documentaire

article

Types déclarés par les sources

article

Éditeurs

Springer Nature

Les mentions d’entités ne constituent pas une preuve de reconnaissance juridique. La juridiction étudiée est distincte du pays d’affiliation des auteurs.

Sources

OpenAlex · Document 1

• Document · https://doi.org/10.1038/s44320-026-00235-4

Statut documentaire

Pertinence à examiner

Bibliothèque

Cette identité n’est pas encore intégrée à la Bibliothèque juridique.